The kit provides a fast, simple and cost-effective purification of plasmid DNA from bacterial cultures. Plasmid DNA can be purified from 1-5 mL of overnight cultures of E. coli. The plasmid DNA isolated by Pure-NA™ Biospin Plasmid Miniprep kit is ready for use in downstream applications such as restriction enzyme digestion, sequencing, PCR/Real-time PCR and other downstream experiments.