Revvity’s Pin-point base editing technology is a three-component system featuring a nickase Cas9 which recruits the effector deaminase through fusion to an aptamer-binding protein. The aptamer is linked to the guide RNA associated with the nuclease in an extended, chimeric RNA scaffold. The modular nature of effector recruitment means that nCas9 can be used for the knockout of one or more gene targets while simultaneously inserting a transgene.

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