The Dharmacon™ CRISPRmod platform of gene modulation tools promote (CRISPRa) or repress (CRISPRi) target gene transcription, without cutting the DNA.

Both CRISPRmod systems (CRISPRa and CRISPRi) use a deactivated Cas9 construct fused to either activators or repressors, to leverage the power of PAM-anchored targeting for transcriptional modulation. CRISPRmod simply brings the precision of CRISPR to gene modulation.

The native Cas9 DNA-cutting functionality has been obliterated by point mutations in the RuvCI and HNH nuclease domains (W. Cheng et al.), changing the protein to a deactivated, or dead, Cas9 (dCas9). This dCas protein is further engineered for targeted transcriptional modulation through the fusing of activator or repressor domains to the C-terminus.

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